Last data update: 24 January 2024 16:39 CET
Host name: Escherichia coli K12 GM48 (LMBP 877)
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| Species: | Escherichia coli |
| Group: | K12 |
| Strain designation: | GM48 |
| Accession number: | LMBP 877 |
| Depositor: | This host strain was deposited by Prof. Dr R. Beyaert1 2. It was obtained from Dr M. Marinus 3. 1 VIB-UGent Center for Inflammation Research, VIB, Ghent, Belgium 2 Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium 3 Department of Pharmacology, University of Massachusetts Medical School, Worcester, USA |
| Containment level: | This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification). |
| Other culture collection numbers: | CGSC 5127 DSM 18040 |
| Medium: | LB-Lennox |
| Selection marker: | / |
| Temperature: | 37°C |
| Genotype: | F- λ- thr-1 araC14 leuB6(Am) fhuA31 lacY1 tsx-78 glnX44(AS) galK2(Oc) galT22 dcm-6 dam-3 thiE1 (Source: CGSC 5127) |
| Phenotype: | - |
| Properties: | - |
| Further information: | This strain carries dam-3, a rather unstable allele. If dam- is required together with the dcm- allele, dam-4 or dam-13::Tn9 would be more recommended. An alternative strain would be Escherichia coli K12 GM2198 (LMBP 4435) which is dcm-6 and dam-13::Tn9. |
| Original reference: | Marinus, Mol. Gen. Genet. 127 (1973), 47-55 [PMID: 4589344] [DOI: 10.1007/BF00267782] |
| Restricted use: | - BCCM MTA - Acknowledge Dr Martin Marinus as originator of the strain |
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Refer in your Materials and Methods: |
Escherichia coli K12 GM48 (LMBP 877) is available at BCCM/GeneCorner. It was deposited by Prof. Dr R. Beyaert and was published in Marinus, 1973. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.